Department of Medical Informatics
University Medical Center Göttingen
Imprint
View primary antibody
Basic data
| PID | primary-0230 |
| EPIC PID | primary-0230 |
| Research group | RG Dobbelstein |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | TP53 |
| Antibody Registry ID(s) | AB_628082 |
| Name | Anti-p53 Antibody (DO-1) |
| Alternative name | LFS1 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | mouse |
| Reacts with | Mouse, Rat, Human |
| Clone | DO-1 |
| Isotype | IgG2a κ |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin |
| Crafted By | company |
| Company / Manufacturer | Santa Cruz Biotechnology |
| Catalog no. | sc-126 |
| Lot no. | |
| Description | Western Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 μg per 1 x 106 cells). |
| Localization | |
| Storage instruction | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Receipt date | 2026-03-09 00:00:00 |
| Preparation date | 2026-03-09 00:00:00 |
| Created by | , |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=TP53 |
| Wikipedia | http://en.wikipedia.org/wiki/TP53 |
| Uniprot | http://www.uniprot.org/uniprot/?query=TP53 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=TP53 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_628082 |
Applications
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Linked publications
The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress
Jansen J, Bohnsack KE, Böhlken-Fascher S, Bohnsack MT, Dobbelstein M
Cell Rep 2024 : 10.1016/j.celrep.2024.114610