View primary antibody

Basic data

PID primary-0230
EPIC PID
Research group RG Dobbelstein
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol TP53
Antibody Registry ID(s) AB_628082
Name Anti-p53 Antibody (DO-1)
Alternative name LFS1
Lab ID
Tag / Fluorophore
Raised in
Reacts with Mouse, Rat, Human
Clone DO-1
Isotype IgG2a κ
Clonality monoclonal
Demasking unknown
Antigen N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
Crafted By company
Company / Manufacturer Santa Cruz Biotechnology
Catalog no. sc-126
Lot no.
Description Western Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 μg per 1 x 106 cells).
Localization
Storage instruction Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
Receipt date
Preparation date
Created by
Last modified

External links

Applications


No application comments yet.

Linked publications


The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress
Jansen J et al.
Cell Rep 2024: 10.1016/j.celrep.2024.114610