View primary antibody

Basic data

PIDprimary-0230
EPIC PIDprimary-0230
Research groupRG Dobbelstein
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTP53
Antibody Registry ID(s)AB_628082
NameAnti-p53 Antibody (DO-1)
Alternative nameLFS1
Lab ID
Tag / Fluorophore
Raised inmouse
Reacts withMouse, Rat, Human
CloneDO-1
IsotypeIgG2a κ
Clonalitymonoclonal
Demaskingunknown
AntigenN-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
Crafted Bycompany
Company / ManufacturerSanta Cruz Biotechnology
Catalog no.sc-126
Lot no.
DescriptionWestern Blotting (starting dilution 1:200, dilution range 1:100- 1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50- 1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 μg per 1 x 106 cells).
Localization
Storage instructionStore at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
Receipt date2026-03-09 00:00:00
Preparation date2026-03-09 00:00:00
Created by,
Last modified,

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Linked publications


The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress
Jansen J, Bohnsack KE, Böhlken-Fascher S, Bohnsack MT, Dobbelstein M
Cell Rep 2024 : 10.1016/j.celrep.2024.114610