View primary antibody

Basic data

PIDprimary-0011
EPIC PIDprimary-0011
Research groupRG Papantonis
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolTUBA4A
Antibody Registry ID(s)AB_2241126
NameAnti-alpha Tubulin antibody
Alternative nameALS22 antibody
Lab ID
Tag / Fluorophore
Raised inmouse
Reacts withmouse, rat, human
CloneDM1A
IsotypeIgG1
Clonalitymonoclonal
Demaskingunknown
AntigenFull length native protein (purified) corresponding to Chicken alpha Tubulin.
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab7291
Lot no.
DescriptionMBExC: primary-0226
Flow Cyt Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. ICC/IF Use a concentration of 0.5 - 1 µg/ml. IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking
Localization
Storage instruction
Receipt date2024-01-16 00:00:00
Preparation date2024-01-16 00:00:00
Created byKusch, Harald
Last modifiedKusch, Harald

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Linked publications


Rapid and synchronous chemical induction of replicative‐like senescence via a small molecule inhibitor
Palikyras S, Sofiadis K, Stavropoulou A, Danieli‐Mackay A, Varamogianni‐Mamatsi V, Hörl D, Nasiscionyte S, Zhu Y, Papadionysiou I, Papadakis A, Josipovic N, Zirkel A, O'Connell A, Loughran G, Keane J, Michel A, Wagner W, Beyer A, Harz H, Leonhardt H, Lukinavicius G, Nikolaou C, Papantonis A
Aging Cell 2024 : 10.1111/acel.14083

Phosphorylation of P-stalk proteins defines the ribosomal state for interaction with auxiliary protein factors
Filipek K, Blanchet S, Molestak E, Zaciura M, Wu CC, Horbowicz-Drożdżal P, Grela P, Zalewski M, Kmiecik S, González-Ibarra A, Krokowski D, Latoch P, Starosta AL, Mołoń M, Shao Y, Borkiewicz L, Michalec-Wawiórka B, Wawiórka L, Kubiński K, Socała K, Wlaź P, Cunningham KW, Green R, Rodnina MV, Tchórzewski M
EMBO Rep 2024 : 10.1038/s44319-024-00297-1